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ISO 6461-1:1986 Water quality -- Detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) -- Part 1 : Method by enrichment in a liquid medium
standard by International Organization for Standardization, 02/01/1986
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The procedure is applicable to all types of water, including turbid water. The principle covers several steps from selection by applying heat to destroy vegetative bacteria to the indication by inoculating volumes of the sample into media followed by incubation at 27 C in anaerobic conditions.