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ISO 6225-2:1990 Rubber, raw, natural - Determination of castor oil content - Part 2: Determination of total ricinoleic acid content by gas chromatography
standard by International Organization for Standardization, 12/01/1990
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Applicable to all grades of natural rubber. The principle is based on extraction of any free ricinoleic acid and conversion to the acetate of methyl ricinoleate. Extraction of any castor oil glycerides also present, hydrolysis to ricinoleic acid, and conversion to the corresponding acetate of methyl ricinoleate. Determination of the total acetate by gas chromatography by comparison either with a ricinoleic acid standard, or with ricinoleic acid prepared by hydrolysis of a castor oil standard.