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ISO 5509:2000
M00008247
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ISO 5509:2000 Animal and vegetable fats and oils -- Preparation of methyl esters of fatty acids
standard by International Organization for Standardization, 04/01/2000
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This International Standard specifies methods of preparing the methyl esters of fatty acids.
It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, three methylation methods are specified, as follows:
boron trifluoride (BF3) method (see clause 3);trimethylsulfonium hydroxide (TMSH) method (see clause 4);trans-esterification method (see clause 5).Methyl esters so produced are used in various analytical procedures requiring such derivatives, for example gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR).