BS PD ISO/TS 21569-5:2016 Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products
BS PD ISO/TS 21569-5:2016 specifies a procedure for the detection of a DNA sequence used in genetically modified(GM) plants by means of a real-time PCR (polymerase chain reaction). The method detects a 78 basepairs long segment of the Figwort mosaic virus 34S promoter DNA sequence. This segment in some GMplants is indicated as FMV promoter (P-FMV) and in other GM plants as FMV enhancer (E-FMV).
The method was developed and validated for the analysis of DNA extracted from foodstuffs. It may besuitable also for analysis of other products such as feedstuffs and seeds. The procedure requires theextraction of an adequate quantity and quality of amplifiable DNA from the test sample.
The DNA sequence amplified by the P-FMV element-specific method can be detected in samples whichcontain DNA of the naturally occurring Figwort mosaic virus. For this reason, it is necessary to confirma positive screening result. Further analyses are required using construct-specific or event specificmethods.
Cross References: ISO 21569 ISO 21570 ISO 21571:2005 ISO 24276 ISO/IEC 17025 ISO 16577
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Product Details
Published: 11/30/2016 ISBN(s): 9780580939433 Number of Pages: 18 File Size: 1 file , 1.9 MB Same As: ISO/TS 21569-5:2016 Product Code(s): 30340144, 30340144, 30340144